The image formats supported by AIJ are the same as the ones
described here for base ImageJ:
http://rsbweb.nih.gov/ij/docs/guide/146-7.html
I am still not sure of the data format of your 32-bit tif image
(RGB, 32-bit floating point, 32-bit integer). The first two are
directly supported by ImageJ/AstroImageJ.
In any supported data format, the intensity measurement should be
valid. RGB "sub-pixels" are combined into a single value for
intensity calculations.
If you would like for me to examine your image to determine
compatibility, please forward it directly to karenacollins ~~~at~~~
outlook ~~~dot~~~ com. In that case it will not show up here in the
public forum.
Karen
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NAML